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摘要：Application of exogenous double-stranded RNA(dsRNA)for virus resistance in plants represents a very attractive alternative to virus resistant transgenic crops or pesticides targeting virus vectors.However,the instability of dsRNA is a major challenge as spraying naked dsRNA onto plants provides protection against homologous viruses for only five days.Innovative approaches,such as the use of nanoparticles as carriers of dsRNA for improved stability and sustained release,are emerging as key disruptive technologies.Knowledge about the mechanism of entry,transport and processing of exogenously applied dsRNA in plants is still limited.In addition,cost of dsRNA and regulatory framework are still key influencers towards practical adoption of this technology.